检测和定量少量 RNA 对于各种生物学应用极为重要,例如在进行 Northern 印迹分析、S1 核酸酶测定、RNase 保护测定、cDNA 文库制备、逆转录之前测量体外转录 RNA 的产量和测量 RNA 浓度PCR、差异显示PCR。测量核酸浓度常用的技术是测定 260 nm 处的吸光度。基于吸光度的方法受到蛋白质、游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。使用敏感且选择性的荧光核酸染色剂可以缓解这种干扰问题。 StrandBrite™ RNA 定量试剂是一种超灵敏荧光核酸染色剂,用于定量溶液中的 RNA。 StrandBrite™ RNA 定量试剂可使用 CytoCite™ 或 Qubit™ 荧光计检测低至 5 ng/mL 的 RNA。我们的 StrandBrite™ Green 荧光 RNA 定量试剂盒包括我们的 StrandBrite™ Green 核酸染色剂和优化且稳定的实验方案。它提供了一种定量溶液中 RNA 的便捷方法。。
参考文献
Oncologic Implications of Chronic Hepatitis C Virus Infection.
Authors: Hwang, Jessica P and LoConte, Noelle K and Rice, John P and Foxhall, Lewis E and Sturgis, Erich M and Merrill, Janette K and Torres, Harrys A and Bailey, Howard H
Journal: Journal of oncology practice (2019): 629-637
Outcomes and costs of single-step hepatitis C testing in primary care, Birmingham, United Kingdom.
Authors: Munang, M and Smit, E and Barnett, T and Atherton, C and Tahir, M and Atabani, S F
Journal: Public health (2019): 40-44
Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots.
Authors: Weber, Jenna and Sahoo, Malaya K and Taylor, Nathaniel and Shi, Run-Zhang and Pinsky, Benjamin A
Journal: Journal of clinical microbiology (2019)
PARP-1/2 Inhibitor Olaparib Prevents or Partially Reverts EMT Induced by TGF-β in NMuMG Cells.
Authors: Schacke, Michelle and Kumar, Janani and Colwell, Nicholas and Hermanson, Kole and Folle, Gustavo A and Nechaev, Sergei and Dhasarathy, Archana and Lafon-Hughes, Laura
Journal: International journal of molecular sciences (2019)