在进行各种分析的DNA样品制备中,DNA定量是一项非常重要的任务。 Portelite 荧光DNA定量试剂盒提供了使用Qubit荧光仪操作Helixyte Green BR快速定量dsDNA的方法。它针对Cytocite 和Qubit 荧光仪进行了优化。 Portelite 荧光DNA定量测定在三个数量级上呈线性。该测定法对RNA上的双链DNA(dsDNA)具有高度选择性,并针对测量10 pg / µL至10 ng / µL的dsDNA浓度进行了优化。Helixyte Green-BR与dsDNA结合后表现出较大的荧光增强,并且比UV吸光度读数高几个数量级。百萤生物是AAT Bioquest的中国代理商,为您提供的Helixyte 绿色双链DNA定量检测试剂盒。
参考文献
A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads
Authors: Choi, Y., Schmidt, C., Tinnefeld, P., Bald, I., Rodiger, S.
Journal: Sci Rep (2019): 4769
A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
Authors: Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.
Journal: Sci Rep (2019): 8853
Effects of Quantification Methods, Isolation Kits, Plasma Biobanking, and Hemolysis on Cell-Free DNA Analysis in Plasma
Authors: Streleckiene, G., Forster, M., Inciuraite, R., Lukosevicius, R., Skieceviciene, J.
Journal: Biopreserv Biobank (2019): ersion="1.0" encoding="UTF-8" ?>17645.enlEndNote1117Streleckiene, G.Forster, M.Inciuraite, R.Lukosevicius, R.Skieceviciene, J.1Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 2Institute of Clinical Molecu