钙离子荧光探针Cal Red R525/650 potassium salt

细胞内钙通量测定法是监测GPCR和钙通道活性的一种广泛使用的方法。为了定量细胞内钙浓度，优选比例荧光荧光指示剂，因为该比率与钙浓度直接相关并且与细胞数和染料负载浓度无关。但是，流行的比例钙指示剂（例如Fura-2和Indo-1）具有一定的局限性，例如灵敏度较低，紫外线激发，并且与HTS筛选滤光片组不兼容。Cal Red R525 / 650已开发为新型的488 nm可激发比例荧光钙指示剂。Cal Red R525 / 650在488 nm处受到很好的激发，在525 nm和650 nm处有两次发射。结合钙后 当在488 nm激发时，Cal Red R525 / 650的发射信号在525 nm处增加，在650 nm处降低。Cal Red R525 / 650的激发和发射波长与普通滤光片组兼容，对细胞的损害小，使其成为评估和筛选GPCR激动剂和拮抗剂以及钙通道靶标的强大工具

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