Fura-10，钾盐

与Fluo-4等单波长探针不同，对于Ca2+，Fura探针的大吸收（或荧光激发）从380 nm（Fura-2），415 nm（Fura-8和Fura-10）转变，与Ca2+结合的无螯合剂可达到约340 nm（Fura-2），355 nm（Fura-8和Fura-10），大荧光发射的波长相对独立于Ca2+浓度。通过使用在340nm和380nm（对于Fura-2），355nm和415nm（对于Fura-8和Fura-10）进行激发并分配荧光强度来获得Ca2+依赖性荧光信号的大动态范围在〜510 nm（Fura-2），525 nm（Fura-8和Fura-10）处检测到。根据该比率，可以使用从校准曲线得出的解离常数（Kd）估算细胞内Ca2+的水平。通过使用在两个波长下激发产生的荧光强度之比，诸如染料分布不均匀和光致漂白等因素将被小化，因为它们应在相同程度上影响两种测量。校准溶液初应不含重金属离子，例如锰，这可能会影响其荧光和对钙的亲和力。百萤生物是AAT Bioquest的中国代理商，为您提供的Fura-10，钾盐。

参考文献

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells.
Authors: Lee, Jeong Hoon and Ha, Jeong Mi and Ho, Quynh Mai and Leem, Chae Hun
Journal: Journal of visualized experiments : JoVE (2019)

Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2.
Authors: Johnson, Martin
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 163-172

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line.
Authors: Malaiyandi, Latha M and Sharthiya, Harsh and Barakat, Ameir N and Edwards, Joshua R and Dineley, Kirk E
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2019): 951-964

A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision.
Authors: Tinning, P W and Franssen, A J P M and Hridi, S U and Bushell, T J and McConnell, G
Journal: Journal of microscopy (2018): 212-220

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-fura-2 and Analogues.
Authors: Zhang, Guangqian and Jacquemin, Denis and Buccella, Daniela
Journal: The journal of physical chemistry. B (2017): 696-705