钙离子荧光探针Cal-520N, AM

钙离子荧光探针Cal-520N, AM是美国AAT Bioquest生产的用于标记钙离子的荧光探针，Cal-520®提供强大的均匀荧光测定工具，用于检测细胞内钙动员。Cal-520®AM是一种新型荧光钙敏感染料，与现有的绿色钙指示剂（如Fluo-3 AM和Fluo-4 AM）相比，具有显着信噪比和细胞内保留。表达通过钙信号传导的GPCR或钙通道的细胞可以预装载可以穿过细胞膜的Cal-520 AM。一旦进入细胞内，Cal-520 AM的亲脂性阻断基团被酯酶裂解，产生带负电荷的荧光染料，留在细胞内。与钙结合后，其荧光大大增强。当用激动剂刺激细胞时，该受体表示细胞内钙的释放，这显着增加了Cal-520®的荧光。Cal-520®AM具有长波长，高灵敏度和> 100倍荧光增强的特性，是测量细胞钙的理想指示剂。高信噪比和更好的细胞内保留使Cal-520®钙测定成为评估GPCR和钙通道靶标以及筛选其激动剂和拮抗剂的有力工具。百萤生物是AAT Bioquest 的中国代理商，为您提供的钙离子荧光探针

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