|
Ex (nm)
|
493
|
Em (nm)
|
515
|
|
分子量
|
-
|
溶剂
|
-
|
|
存储条件
|
在零下15度以下保存, 避免光照
|
Screen Quest Calbryte 520 免丙磺舒和免洗钙检测试剂盒是美国AAT Bioquest研发的用于检测钙离子的试剂盒钙通量测定是用于筛选G蛋白偶联受体（GPCR）的药品发现中的优选方法。 Screen Quest Calbryte-520不含Probenecid和Wash-Free的钙测定试剂盒提供强大的均相荧光测定，用于检测细胞内钙动员。表达感兴趣的GPCR的细胞通过钙预先加载我们专有的Calbryte -520NW，其可以穿过细胞膜。 Calbryte -520 NW是适合HTS筛查的钙指示剂。一旦进入细胞内，Calbryte -520NW的亲脂性阻断基团被非特异性细胞酯酶切割，导致带电荷的荧光染料停留在细胞内，并且在与钙结合后其荧光大大增强。当用筛选化合物刺激细胞时，该受体表明细胞内钙的释放，这极大地增加了Calbryte -520NW的荧光。其优异的细胞保留特性，高灵敏度和100-250倍的荧光增加（当它与钙形成复合物时）使Calbryte -520NW成为测量细胞钙的理想指标.Calbryte -520NW是钙染料不需要丙磺舒以获得更好的细胞保留。这款Screen Quest Calbryte-520不含Probenecid和Wash-Free的钙测定试剂盒提供了优化的检测方法，用于监测G蛋白偶联受体（GPCR）和钙通道与脆弱或困难的细胞系。该测定可以以方便的96孔或384孔微量滴定板形式进行，并且易于适应自动化。百萤生物是AAT Bioquest 的中国代理商，为您提供的钙检测试剂盒
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