Screen Quest 免洗Fura-2钙检测试剂盒

钙通量分析是筛选G蛋白偶联受体（GPCR）药品发现的方法。该比值钙测定试剂盒允许均匀测量由G蛋白偶联受体或钙通道引起的细胞内钙变化。表达通过钙传递信号的GPCR的细胞被Fura-2AM预加载，Fura-2AM可以穿过细胞膜。一旦进入细胞内，亲脂封闭基团Fura-2AM被酯酶裂解，导致带负电荷的荧光染料留在细胞内，其荧光波长在与钙结合时蓝移。当细胞受到激动剂刺激时，受体发出细胞内钙离子释放的信号，从而大大提高Fura-2在短波处的荧光强度。Fura-2的比色特性使该试剂盒成为比单波长Fluo-4测定细胞钙浓度的理想工具。通过单次添加，该分析很容易在高透性环境中进行。该方法可用于96孔或384孔微孔板，且易于自动化。百萤生物是AAT Bioquest的中国代理商，为您提供的Screen Quest 免洗Fura-2钙检测试剂盒

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