Screen Quest cAMP检测试剂盒*荧光ELISA法*

Screen Quest 荧光ELISA cAMP分析试剂盒提供了一种优化的分析方法，用于检测G蛋白偶联受体系统中腺苷酸环化酶的。该测定基于HRP标记的cAMP与未标记的cAMP之间固定数目的抗体结合位点的竞争。通过未标记的游离cAMP将HRP-cAMP从HRP-cAMP /抗cAMP抗体复合物中置换出来。在不存在cAMP的情况下，HRP-cAMP偶联物仅与抗cAMP抗体结合。然而，测试样品中未标记的游离cAMP与HRP-cAMP抗体偶联物竞争抗cAMP抗体，因此抑制了HRP-cAMP与抗cAMP抗体的结合。我们的Screen Quest 荧光cAMP分析试剂盒提供了检测腺苷酸环化酶活性的灵敏方法。与其他商用ELISA cAMP分析试剂盒相比，它繁琐的乙酰化步骤。该试剂盒使用Amplite Red作为荧光HRP底物来定量HRP活性。形成的荧光产物与HRP-cAMP偶联物的活性成比例。百萤生物是AAT Bioquest的中国代理商，为您提供的Screen Quest cAMP检测试剂盒。

参考文献

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Role of the cAMP Pathway in Glucose and Lipid Metabolism
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Journal: Handb Exp Pharmacol (2016): 29