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- 产品名称：AAT Bioquest HDAC活性检测试剂盒
- 产品型号：13601
- 产品展商：AAT Bioquest
- 产品文档：无相关文档
简单介绍
AAT Bioquest HDAC活性检测试剂盒，蛋白去乙酰化酶（histone deacetylase,HDAC）是一类蛋白酶，对染色体的结构修饰和基因表达调控发挥着重要的作用。在癌细胞中，HDAC的过度表达导致乙酰化作用的增强，通过恢复组蛋白正电荷， 从而增加DNA与组蛋白之间的引力，AAT Bioquest HDAC活性检测试剂盒，很受欢迎。
产品描述
产品描述：
蛋白去乙酰化酶（histone deacetylase,HDAC）是一类蛋白酶，对染色体的结构修饰和基因表达调控发挥着重要的作用。在癌细胞中，HDAC的过度表达导致乙酰化作用的增强，通过恢复组蛋白正电荷， 从而增加DNA与组蛋白之间的引力， 使松弛的核小体变得十分紧密， 不利于特定基因的表达， 包括一些肿瘤抑制基因。组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitors，HDACi）则可通过提高染色质特定区域组蛋白乙酰化，从而调控细胞凋亡及分化相关蛋白的表达和稳定性，诱导细胞凋亡及分化，成为一类新的抗肿瘤**。
产品性能：
Amplite HDAC检测试剂盒提供了一种快速、便捷、灵敏的方法来检测HDAC的活性。我们的HDAC绿色底物是一种非肽类复合物，它比其它基于肽类的HDAC底物更加稳定。我们的试剂盒可用于检测细胞裂解液中HDAC的活性，也可以与配合抽提物或纯化的酶应用于体外抑制剂的筛选。HDAC Green绿色荧光底物的长波长发射光合高激发系数，这使其在检测中不会引起很大的干扰。HDAC活性的测定，通过检测490nm处激发光和520nm处发射光的绿色荧光增强来实现。
英文注释：
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction. It regulates blood pressure and electrolyte homoeostasis. Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. Renin has been identified to be an attractive target for the treatment of hypertension. The Amplite Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and renin activity using our proprietary Tide Fluor 3 (TF3)/Tide Quencher 3 (TQ3) fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, and the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay. With the Amplite Renin Assay Kit, we have detected as little as 1ng renin in a 100 reaction volume.
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